Uncária tomentosa no tratamento da mucosite intestinal induzida por metotrexato em camundongos

Abstract

To investigate the effect of supplementation of Uncaria tomentosa in the small intestine of mice with intestinal mucositis. The experiment lasted 5 days and used 24 male mice of the Swiss strain, with an average age of two months. The animals were kept in a controlled ambient temperature of 25 ± 2°C and 12 hour photoperiod. For the diet was used standard commercial rodent. The animals were divided into group 1 (control with the offer of commercial feed and water at libitum; group 2 (mucositis), subjected to induction of intestinal mucositis by methotrexate in the past 3 consecutive days of the experiment and group 3 (Uncaria tomentosa) dfoi administered by gavage extract containing 0,536g of Uncaria tomentosa. GROUP 4 (Uncaria tomentosa + mucositis) had the same procedure of the previous group with the methotrexate drug addition in the last 3 days. the animals were sacrificed by cervical dislocation and analysis histomorphometric were collected 3 cm of the duodenum to analyze the morphology of duodenal epithelium. it was noted that group 4 had the highest weight loss (1.5g) when compared with the other group. in evaluating the villi, it was observed that the group mucositis, had the smallest length of villi in the other groups. the Uncaria tomentosa group had intestinal villi and crypts with shallower compared to the control group. Comparing the Uncaria tomentosa, with the other groups, there was an increase in the size of villus height. Regarding the depth of the crypts, it was observed that the Uncaria tomentosa group had lower average compared to other groups and mucositis group had higher average depth of the crypts. The U. tomentosa promoted weight loss and recovery of the intestinal mucosa of animals with mucositis.